BCH310 TUTORIAL QUESTIONS

UNIVERSITY OF BUEA ONLINE STUDENT ASSOCIATION (UBOSA) COORDINATOR: LOKENDO T.                                                                       DEPT: BIOCHEMISTRY AND MOLECULAR BIOLOGY COURSE CODE: BCH 310                                                                       COURSE TITLE     GENETICS TUTORIAL SESSION 5                                                                                         Date: Thursday 7th May 2015      Time: 8-11am Motto: Thinking out of the box for excellence

 

1)      What do you understand by each of the following?

     I.            Chromosomal aberration.

   II.            Synonymous mutation.

2)      What is genetics? Give importance of genetics.

3)      Distinguish between the following pairs

     I.            Euchromatin and heterochromatin.

   II.            Constitutive and facultative heterochromatin.

4)      What do you understand by the melting point of DNA?

5)      What is the melting point of a double stranded DNA molecule that contains

     I.            27% GC?

   II.            56% GC?

6)      Name the major helical conformations of DNA.

7)      What do you understand by major and minor grooves with respect to DNA structure?

8)      What are the Watson and Crick base pairs? Why are they important?

 

9)      Consider the following molecules: Guanine (G); Adenine(A); Uracil(U); Thymine(T); Cytosine(C) and the following properties:

                    I.            Possesses at least a hydroxyl function.

                  II.            Possesses an amine function.

                III.            Does not contain an oxygen atom.

                IV.            Possesses a methyl group

                  V.            Can base pair wit adenine

                VI.            Is a purine base.

              VII.            Is a pyrimidine base.

Associate to each molecule, the number(s) of the property(s) that is (are) applicable to it.

10)   Is the intracellular messenger cAMP a nucleoside or a nucleotide?

11)   Match the following list of scientists against their discoveries

Scientists	Discoveries
12)   Darwin and Wallace	I.            Genes linearly arranged on some chromosomes and mapped.
13)   TH Morgan	II.            Related “genes” to enzymes and biochemical processes
14)   Sutton Boveri	III.            Chromosomal theory of inheritance
15)   Gregor Mendel	IV.            Theories of acquisition of inheritable traits
16)   Friedrich Miescher	V.            Theories of evolution
17)   Beadle and Tatum	VI.            Came up with a central dogma (DNAàmRNAàProteins)
18)   AH Sturtevant	VII.            Theories of transmission of traits
19)   Oswald Avery x	VIII.            Identified DNA in 1869
20)   Watson, Crick, Franklin & Wilkins	IX.            Genes on chromosomes
21)   Lamarck	X.            DNA was the genetic material

22)   Give one example of each mutagens and briefly describe how it increases the rate of mutagenesis:

        I.            An intercalating agent

      II.            A DNA-modifying chemical

    III.            A base analog

23)   a) If the GC content of a DNA molecule is 24%, what are the percentages of the four bases (A, G, C, and T) in the molecule?

             b)  What is the melting temperature of this DNA?

Good Luck

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